Efek Pemberian Ekstrak Etanol Daun Dan Batang Ubi Jalar Ungu (Ipomoea batatas L.) Terhadap Penurunan Kadar Glukosa Darah Pada Tikus Jantan Galur Wistar Yang Diinduksi Aloksan:

The effects of Ethanol Extract of Leaves and Stems of Purple Sweet Potatoes (Ipomoea batatas L.) on Decreasing Blood Glucose Levels in Aloxan-Induced Wistar Male Rat

Abstract

Tanaman ubi jalar ungu (Ipomoea batatas L.) mudah didapat dan banyak kegunaannya,terutama bagian umbi ubi jalar ungu yang banyak diolah masyarakat sedangkan bagian daun dan batang pemanfaatnya belum maksimal. Umbinya telah diketahui mempunyai aktivitas dapat menurunkan kadar gula darah. Penelitian ini bertujuan untuk mengetahui efek penurunan kadar glukosa darah dari ekstrak etanol daun dan batang ubi jalar ungu serta golongan senyawa yang terkandung dalam ekstrak. Desain penelitian menggunakan randomized pre-test post-test kontrol grup desain. Pada penelitian ini menggunakan 15 ekor tikus jantan galur wistar dan dibagi menjadi 5 kelompok yaitu kontrol negatif (akuades), kontrol positif (metformin 45 mg/kgBB), kelompok perlakuan ubi jalar ungu dosis 150; 300; 600 mg/kgBB, sebelumnya masing –masing tikus dinduksi dengan aloksan 150 mg/kgBB, dan dilakukan pemantauan selama 14 hari. Pemeriksaan kadar gula darah menggunakan spektrofotometer visibel dengan panjang gelombang 500 nm. Data dianalisis secara statistik untuk mengetahui perubahan kadar gula darah. Hasil yang didapat dengan pemberian ekstrak pada dosis 150, 300, dan 600 mg/kgBB selama pemantauan 14 hari dapat menurunkan kadar gula darah berturut – turut menjadi 96,33 ± 4,72 ; 66,67 ± 16,16 ; dan 142,67 ± 27,06 mg/dL. Ekstrak etanol daun dan batang ubi jalar ungu memiliki kandungan senyawa yaitu alkaloid, flavonoid, saponin, tanin dan senyawa fenolik.

The purple sweet potato plant (Ipomoea batatas L.) is easy to obtain and has many uses, especially the purple sweet potato tubers which are mostly utilized by the people, while the utilization of its leaves and stem are not maximized. Its tuber has been known to have activity in  reducing blood sugar levels.This research aimed to evaluate the effect of ethanol extract of leaves and stems of purple sweet potatoes in reducing blood glucose level as well as to identify its chemical constituents. The study was design by a randomized pre-test post-test control group. The animal used were 15 wistar male rats which divided into 5 groups, these include negative control (distilled water), positive control (metformin 45 mg/kgBW), treatment group of purple sweet potatoes with the doses of 150; 300; 600 mg/kgBW, before the treatment all rats were induced by aloxan 150 mg/kgBW, and all groups were observed for 14 days.

Blood glucose level was determined using UV-Vis spectophotometer at wavelength of 500 nm. The data were analyzed statistically to evaluate blood glucose level alteration.  The result showed that the extract at doses of 150, 300, dan 600 mg/kgBW decrease blood glucose level in 14 days observation, the blood glucose levels were 96.33 ± 4.72 ; 66.67 ± 16.16 ; and  142.67 ± 27.06 mg/dL, respectively. The ethanol extract of leaves and stem of purple sweet potatoescontained alkaloids, flavonoids, saponins, tannins and phenolic compounds