Pengamatan Tingkat Variasi Genetik dengan Menggunakan Teknik DNA Profilling pada Tanaman Kelapa Sawit (Elaeis Guineensis Jacq.) Berdasarkan Marka Random Amplified Polymorpism DNA (RAPD):

Observation of the Level of Genetic Variation by Using the technique of DNA Profiling on Palm Oil Plants (Elaeis Guineensis Jacq.) Based on the Random Amplified Polymorphism DNA (RAPD) Markings

Abstract

Proses pemuliaan tanaman merupakan langkah awal dalam mendapatkan varietas unggul tanaman kelapa sawit. Pengamatan terhadap variasi genetik perlu dilakukan untuk menyusun program pemuliaan tanaman. Teknik DNA profiling dapat dilakukan untuk mendeteksi variasi genetik ditingkat molekuler. Tujuan penelitan adalah untuk mendapatkan keragaman molekuler berdasarkan marka RAPD. Metode penelitian dilakukan dengan mengamati persentase filogenetik analysis dan PCOA analysis. Adapun hasil penelitian menunjukkan bahwa populasi membentuk tiga kluster dan berdasarkan analisis PCoA menunjukkan tingkat keragaman molekuler sebesar 43.65%.

The plant breeding process is the first step in getting superior varieties of oil palm plants. Observation of genetic variation needs to be done to develop a plant breeding program. DNA profiling techniques can be carried out to detect genetic variations at the molecular level. The aim of the research to obtain molecular diversity based on RAPD markers. The research method was carried out by observing the percentage of phylogenetic analysis and PCOA analysis. The results showed that the population formed three clusters and based on PCOA analysis showed a level of molecular diversity of 43.65%.